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Fig. 3 | Molecular Cancer

Fig. 3

From: Knockdown of long non-coding RNA NEAT1 inhibits glioma cell migration and invasion via modulation of SOX2 targeted by miR-132

Fig. 3

NEAT1 targets miR-132 and miR-132 targets SOX2. a The relative expression of miR-132 decrease in glioma tissue compared with that in normal tissue. The miR-132 expression is lower in high grade (stage III and stage IV) glioma tissue than that in low grade (stage I and stage II) glioma tissue. U6 is the internal control. * P < 0.05, ** P < 0.01 and *** P < 0.001. b MiR-132 is predicted to be a target miRNA of NEAT1. c The relative luciferase activity in cells co-transfected with miR-132 mimics and NEAT1-wt decrease while that in cells co-transfected with miR-132 mimics and NEAT1-mut has no significant change. ** P < 0.01. NS: not significant. d The relative expression of miR-132 in glioma cells significantly increases in si-NEAT1 group. U6 is the internal control. ** P < 0.01 compared with si-NC group. e SOX2 is predicted to be a target gene of miR-132. f The relative luciferase activity in cells co-transfected with miR-132 mimics and SOX2-wt decreases while that in cells co-transfected with miR-132 mimics and SOX2-mut has no significant change. ** P < 0.01. NS: not significant. g The dose-response diagram shows that the miR-132 inhibitor significantly suppresses miR-132 activity at concentration of 100 nM, 120 nM, 150 nM and 200 nM. We chose 120 nM in this study. h The relative mRNA expression of SOX2 in glioma cells increase in miR-132 inhibitor group and decrease in miR-132 mimics group. GAPDH is the internal control. ** P < 0.01 compared with NC group. i The protein expression of SOX2 in glioma cells increase in miR-132 inhibitor group and decrease in miR-132 mimics group. β-actin is the internal control. Each experiment was performed in triplicate and measured for 3 times. j The histogram shows the expression of SOX2 protein in U251 and U87 cell lines. ** P < 0.01 compared with NC group

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