Fig. 4
From: Suppression of PDHX by microRNA-27b deregulates cell metabolism and promotes growth in breast cancer
PDHX shRNA recapitulates the effects of miR-27b overexpression. a PDHX protein expression in MCF7 (left) and HEK-293Â T cells (right) is reduced most strongly by shRNA 1 and 2. PDH complex activity (b) and DMEM lactate levels (c) following transient expression of shRNA vectors targeting PDHX in MCF7 cells. d Stable transfection using the same shRNA expression vectors was carried in MCF7 cells, which were similarly assessed for changes to PDHX protein. These shRNA cells were assessed for changes in PDH activity (e), lactate (f), pyruvate (g) and citrate (h) levels. Seahorse mitochondrial stress test quantitating OCR (i) and ECAR (J) of MCF7 PDHX KD and control cells