Fig. 6

Signal transduction in the RASAL2-hippo cascade. a Knockdown of RASAL2 significantly increased translocation of YAP1 from the nucleus to cytoplasm in SW620 and Caco2 cells, Blue: Nucleus, Green: YAP1, Red: β-actin. b Immunoblotting with anti-HA antibody showed that more YAP1 proteins were ubiquitinated in siRASAL2 groups when compared to siControl in CRC cells, whereas proteasome inhibitor MG132 treatment lead to no further increase in YAP1 degradation through the proteasome. c IC50 of verteporfin decreased significantly in RASAL2 downregulated group when compared with siControl group in SW620 and Caco2 cells at 24 h after verteporfin incubation. d Nuclear YAP1 negative (Upper) and positive (Lower) using IHC of YAP1 in CRC; Nuclear YAP1 was overexpressed and significantly positively correlated with RASAL2 (Right, P < 0.01). e Overexpression of YAP1 was positively associated with RASAL2 mRNA expression in GENT and TCGA. (*, P < 0.05; **, P < 0.01, Student’s t-test, n = 3 independent experiments)