Fig. 2From: The long non-coding RNA CYTOR drives colorectal cancer progression by interacting with NCL and Sam68CYTOR promotes anchorage-independent growth and migration/invasion in vitro. a CYTOR levels in RKO, SW480, SW620, HCT116 and HCT8 cell lines by qRT-PCR. b Knockdown of CYTOR by shRNA in the RKO, SW480 and SW620 cell lines. c Reduction of colony formation ability for CYTOR knockdown (shRNA) RKO and SW620 cells compared with control (scramble) by soft agar and plates assay (right histogram represents quantification analysis). d Decrease of migration/invasive potential for CYTOR knockdown RKO, SW480 and SW620 cells compared with control by transwell assay (right histogram represents quantification analysis). e qRT-PCR for CYTOR levels in empty-vector (EV) and overexpression-vector (CYTOR)-transfected HCT116 and HCT8 cells. f Increase of colony formation ability for overexpression-vector (CYTOR)- transfected HCT116 and HCT8 cells compared with empty-vector (EV) by plates colony formation assay (Right histogram represents quantification analysis). g Increase of migration/invasive potential for CYTOR overexpression (CYTOR) HCT116 and HCT8 cells compared with empty-vector (EV) by transwell assay (Right histogram represents quantification analysis). h qRT-PCR for CYTOR levels in HCT116 cells with a CYTOR overexpression vector (CYTOR) and co-transfection CYTOR siRNA (CYTOR+siRNA). i Increase of migration/invasive potential for CYTOR-overexpressing (CYTOR, Lane 2) HCT116 cells and rescue potential of CYTOR siRNA (CYTOR+siRNA, Lane 4) by transwell assay. j Expression of E-cadherin and Vimentin in CYTOR knockdown SW480 and SW620 cells by immunoblotting. k Expression of E-cadherin and Vimentin in CYTOR-overexpressing HCT116 and HCT8 cells by immunoblottingBack to article page