Fig. 5

HIF1β transcription activation by KDM4D is closely correlated with H3K9me3 and H3K36me3 demethylation. a Western blot reveals HIF1β and VEGFA protein expression among indicated GIST cells. b, c, d, e Western blot reveals expression of indicated proteins among vector, KDM4D, ShNC and ShKDM4D GIST882 and GSIT-T1 cells (b, d); The right histogram showed that the quantification of related protein (c, e). f CHIP assay was used to examine the interaction between KDM4D and the HIF1β promoter in GIST882 cells. g, i CHIP assays were used to examine the interaction between the HIF1β gene promoter and H3K9me3 and H3K36me3 among indicated cells. h, j Dual-luciferase assays were performed to detect gene luciferase activity of the HIF1β promoter among indicated cells. ***p < 0.001