Fig. 1From: Genome-scale CRISPR-Cas9 knockout screening in gastrointestinal stromal tumor with Imatinib resistanceSchematic and results of functional screening by sgRNA library and imatinib treatment. a Optical microscopic images of GIST-T1 cells treated with imatinib at day 4. b Structure of one vector lentiviralGeCKO system; c Schematic of imatinib-resistant GIST-T1 cells construction for high-throughput sequencing analysis; d Optical microscopic images of GIST-T1cells transfected with lentiviral sgRNA library and treated with imatinib. a-control, b-imatinib (40 μg/mL), c-transfection and imatinib (40 μg/mL)Back to article page