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Fig. 4 | Molecular Cancer

Fig. 4

From: LncRNA PTAR promotes EMT and invasion-metastasis in serous ovarian cancer by competitively binding miR-101-3p to regulate ZEB1 expression

Fig. 4

Silencing PTAR alleviates ovarian cancer cell tumorigenicity. The SKOV3, A2780 and OVCAR3 cells were transfected with sh-PTAR with or without miR-101 inhibition in the presence or absence of 10 ng/ml TGF-β1 for 48 h. a, b Wound healing assays were performed both in SKOV3 and A2780 cells treated as specified. n = 4, *P < 0.05 vs. Ctrl, #P < 0.05 vs. TGF-β1 + sh-Scram, &P < 0.05 vs. TGF-β1 + sh-PTAR. c, d Cell migration in SKOV3 and A2780 cells was determined using transwell chambers. n = 4, ** P < 0.01. e The invasion ability of SKOV3 cells was evaluated using matrigel transwell chambers. n = 4, ** P < 0.01. f, g The protein levels of E-cadherin, vimentin, FN1 and ZEB1 in SKOV3 and A2780 cells. n = 5, *P < 0.05 vs. Ctrl, #P < 0.05 vs. TGF-β1 + sh-Scram, &P < 0.05 vs. TGF-β1 + sh-PTAR. h The protein levels of E-cadherin and ZEB1 in OVCAR3 cells. n = 3, *P < 0.05 vs. Ctrl, #P < 0.05 vs. TGF-β1 + sh-Scram, &P < 0.05 vs. TGF-β1 + sh-PTAR

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