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Fig. 6 | Molecular Cancer

Fig. 6

From: LncRNA PTAR promotes EMT and invasion-metastasis in serous ovarian cancer by competitively binding miR-101-3p to regulate ZEB1 expression

Fig. 6

Silencing miR-101 promotes OvCa cell migration by regulating ZEB1. a The western blot analysis of epithelial and mesenchymal markers in SKOV3 cells transfected with AMO-101. Wound-healing b and migration assays c were applied to determine the effect of miR-101 knockdown on OvCa cell migration. d The luciferase reporter activity of chimeric vectors carrying the luciferase gene and a fragment of the ZEB1 3′-UTR containing the wild-type or mutated miR-101 binding site. e The overexpression of miR-101 inhibited the mRNA expression of ZEB1 in SKOV3 cells. f The overexpression of miR-101 repressed the protein expression of ZEB1 in SKOV3 cells, whereas knockdown of miR-101 had the opposite effect. n = 5. *P < 0.05, **P < 0.01

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