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Fig. 3 | Molecular Cancer

Fig. 3

From: The chromosome 11q13.3 amplification associated lymph node metastasis is driven by miR-548k through modulating tumor microenvironment

Fig. 3

In vivo mouse model studies reveal miR-548k promoted xenograft tumor formation, lymphangiogenesis, lymphatic metastasis and distant metastases. a, Stable overexpression of miR-548k in KYSE30 cells enhanced subcutaneous xenograft tumors formation in BALB/c nude mice (n = 14). Left, representative picture. Right, growth curves of xenograft tumors. b, Stable overexpression of miR-548k in KYSE30 cells promoted subserosa tumor growth in esophageal abdominal portion (n = 6 in miR-548k overexpression group and n = 4 in control group). Representative HE photos of esophageal subserosa tumors. Scale bar: 500 μm or 100 μm. c, Representative images and quantitative analysis of the popliteal lymph nodes immunostained with anti-GFP antibody (*p < 0.05, t-test). Scale bar: 500 μm or 100 μm. d, Representative images of peri-tumoral and intra-tumoral sections immunostained with anti-LYVE-l antibody (left), and quantification (right), indicates the microlymphatic vessel density (*p < 0.05, t-test). Scale bar: 100 μm. e, Representative images of peri-lymph node and intra-lymph node sections immunostained with anti-LYVE-l antibody (left), and quantification (right), indicates the microlymphatic vessel density (**p < 0.01, ns, no significance, t-test). Scale bar: 100 μm. f, MiR-548k promoted local invasion in subcutaneous xenograft tumors formation model (p = 0.0213, Fisher’s Exact-test). Representative pictures (up) and quantitative data (bottom), T, tumor; M, muscle. Scale bar: 500 μm. g, Stable overexpression of miR-548k in KYSE30 cells promoted the lung metastasis (p = 0.0004, Fisher’s Exact-test). Representative pictures (up) and quantitative data (bottom). Scale bar: 500 μm or 100 μm

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