Fig. 4

SPAG5 is targeted by miR-363-3p. a PLC8024 and QGY-7703 cells were transfected with miR-363-3p mimics for 36 h. The SPAG5 mRNA levels were determined by qRT-PCR. b Cells were treated with miR-363-3p inhibitor for 36 h. The SPAG5 mRNA levels were determined by qRT-PCR. c Proteins from cells treated with miR-363-3p mimics or inhibitors were subjected to western blot to examine the alteration of SPAG5 protein. d Luciferase reporter assays were performed to indicate the effect of miR-363-3p on the activity of SPAG5 mRNA 3’UTR. e The correlation of SPAG5 mRNA and miR-363-3p was determined in 27 HCC samples. f Cells expressing miR-363-3p were transfected with SPAG5 overexpression vector for 24 h. The cell proliferation was examined by foci formation assays. g The impact of SPAG5 in miR-363-3p-mediated cell migration was determined by Transwell assays. h The cell cycle analyses were performed in cells with miR-363-3p and SPAG5. The percentage of cells in G1 phase was indicated. *P < 0.05, **P < 0.01