Fig. 2

Reduced expression of LINC01133 promotes proliferation and migration and induces the EMT in GC cells. a qRT-PCR was conducted to verify the relative expression of LINC01133 in AGS and BGC-823 cells transfected with two independent shRNAs targeting LINC01133. b CCK-8 assay of AGS and BGC-823 cells after knockdown of LINC01133. c, d Representative results of the colony formation and transwell assays of AGS and BGC-823 cells after shLINC01133–1 or shLINC01133–2 transfection. e Representative images of IF micrographs of the subcellular localization and expression of E-cadherin (green) and vimentin (red). Nuclei were counterstained with DAPI (blue). Scale bars represent 50 μm. For all quantitative results, the data are presented as the mean ± SD from three independent experiments. ^*P <  0.05 and ^**P <  0.01