Fig. 5

APC is a direct target of miR-106a-3p in GC metastasis. a Different expression of metastasis-related genes were examined in AGS cells after miR-106a-3p inhibitor or negative control transfection using a human tumor metastasis PCR array. Red marks, > 2-fold change (log2) and P <  0.05 (−log10); Green mark, < − 2-fold change (log2) and P <  0.05 (−log10). b The expression levels of eight upregulated candidate mRNAs confirmed by qRT-PCR in 30 paired GC cancer tissues and matched normal tissues. c Luciferase reporter assay in HEK-293FT cells co-transfected with wide type (WT) or mutated (Mut) APC 3’-UTR reporter vector and miR-106a-3p mimic or inhibitor. d Relative expression of APC mRNA in AGS cells with LINC01133 knockdown or SGC-7901 cells with LINC01133 overexpression. e qRT-PCR was conducted to evaluate the mRNA expression of APC gene in AGS cells following reduced expression of LINC01133 and/or inhibition of miR-106a-3p. f qRT-PCR was performed to access the mRNA expression of APC gene in AGS cells following ectopic expression of miR-106a-3p and/or pCMV-APC expression vector lacking the 3’-UTR. g Representative images and quantification of cell migration in AGS cells after transfection miR-106a-3p mimic and/or pCMV-APC lacking the 3’-UTR. Error bars: mean ± SD, n = 3. ^*P <  0.05 and ^**P < 0.01