Fig. 1

ANRIL is significantly highly expressed in AML patient samples and regulates AML progression in vitro and in vivo. a The expression of ANRIL in AML patients was detected by qRT-PCR, **p < 0.01,***p < 0.001. b Knockdown of ANRIL can induce cell senescence in MOLM-13 cells, ***p < 0.001. c The cell proliferation detected using CCK-8 and Edu assays, respectively, in MOLM-13 lines were blocked when ANRIL knocked down, ***p < 0.001. d ANRIL knocked down enhanced ATO-induced cell apoptosis in a time course (24 h, 48 h, 72 h) in MOLM-13 cells, *p < 0.05, ***p < 0.01. The representative photograph of flow cytometry was shown. e The western blot for the cleaved PARP and caspase 3 upon knockdown of ANRIL in HL60 cells. f The western blot for the expression levels of cleaved PARP and caspase 3 under the overexpression of ANRIL. g NOD-scid-mice model that intravenously (tail vein) implanted by sh-NC and sh-ANRIL established MOLM-13 cells, the percentages of GFP+ MOLM-13 cells were checked in BM, and spleen after 3 weeks implantation. Error bars reflect ±SEM (*, p < 0.05). h Bone marrow smear showed the amount of GFP+ MOLM-13 cells from sh-ANRIL group after transplantation. i Kaplan–Meier curves showed the survival of the sh-NC and sh-ANRIL established mice (*, p < 0.05)