Fig. 4

Effect of exosomal PTENP1 on bladder cancer cellular phenotype. Exosomes (Exos) were isolated from 293A cells transfected with PTENP1-expressing plasmid or NC vector, namely PTENP1-Exos and NC-Exos, respectively. Their exosomes were extracted and added to the EJ and J82 cells for 24 h. a A CCK8 assay detection of cell viability. b qRT-PCR detection of the PTENP1 mRNA level. c A colony-forming growth assay detection of cell colony formation ability. The colonies were counted and captured. d Representative images of invasion assays of EJ (upper) and J82 cells (lower). The number of cells were counted. e Representative images of migration assays of EJ (upper) and J82 cells (lower). The number of cells were counted. f Flow cytometry detection of the apoptosis of EJ (upper) and J82 cells (lower). g Flow cytometry detection of cell cycle of EJ (upper) and J82 cells (lower). Results are presented as mean ± SD. *P < 0.05. All of the experiments were performed in triplicate