Fig. 5
From: A new ALK isoform transported by extracellular vesicles confers drug resistance to melanoma cells
EVs can transfer functional properties. (a) Sensitive A375 melanoma cells were co-cultured with both EV-A375 and EV-A375X1 (10 μg/ml). After 24 h, vemurafenib dose-response analysis was performed to calculate the IC50. Representative dose-response curves of sensitive A375 (black), sensitive A375 plus EV-A375 (grey) and sensitive A375 plus EV-A375X1 (dotted line). (b) PLX4032 IC50 values of sensitive A375 (black), sensitive A375 plus EV-A375 (grey) and sensitive A375 plus EV-A375X1 (white). Error bars represent the standard deviation of three biological replicates. Statistical significance was determined using paired Student’s t-tests. *p < 0.05, **p < 0.01, ***p < 0.001. (c) Venn diagram showing unique and shared proteins identified by mass spectrometry in EVs isolated from both sensitive A375 and resistant A375X1 cells. (d) ALK consensus sequence in which the highlighted peptides are the ones detected by MS in the resistant EVs. (e) ALK western blot analysis of sensitive and resistant cells and corresponding EVs. Results are shown for one representative of three biological replicates