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Fig. 1 | Molecular Cancer

Fig. 1

From: Circular RNA F-circEA-2a derived from EML4-ALK fusion gene promotes cell migration and invasion in non-small cell lung cancer

Fig. 1

Identification of F-circEA-2a in NSCLC. a Schematic representation of F-circEA-2a/4a generated from EML4-ALK gene. The divergent primers (F1/R1) were used to detect F-circEA-2a/4a. b RT-PCR and Sanger sequencing of F-circEA-2a in H2228 cells. The arrow indicates the junction site of F-circEA-2a. c Measurement of F-circEA-2a/4a in H2228 cells by dot blot hybridization (left) and qPCR (right, normalized to GAPDH mRNA). d qPCR analysis of F-circEA-2a after nucleus/cytoplasm fractionation of H2228 cells. GAPDH mRNA and U6 RNA were used to indicate the cytoplasmic and nuclear RNA, respectively. Western blotting confirmed good nucleus/cytoplasm fractionation. Data are shown as the mean ± SD. See Additional file 1: Table S1 for the information of the primers and oligonucleotides in this figure. The experimental protocols are described in the Additional file 2

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Molecular Cancer

ISSN: 1476-4598