Fig. 2

F-circEA-2a promotes cell migration and invasion in NSCLC cells. a Schematic representation of F-circEA-2a-expressing plasmid with the flanking sequence of laccase 2 to facilitate RNA circularization. b Agarose gel electrophoresis and Sanger sequencing of RT-PCR products from H1299 cells transfected with F-circEA-2a-expressing plasmid and empty vector. c.d Nucleus/cytoplasm fractionation and qPCR analysis of F-circEA-2a in A549 (c) and H1299 (d) cells. Western blotting against laminA/C and tubulin showed efficient nucleus/cytoplasm fractionation. Data are shown as the mean ± SD. e.f.g MTT (e.f) and colony formation assays (g) in A549 and H1299 cells transfected with F-circEA-2a-expressing plasmid or empty vector (Ctrl). h.i Representative images of Transwell (h) and wound-healing assays (i) in A549 and H1299 cells transfected with F-circEA-2a-expressing plasmid or empty vector (Ctrl). See Additional file 1:Table S1 for the information of the primers and oligonucleotides in a and b. The experimental protocols are described in the Additional file 2