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Fig. 3 | Molecular Cancer

Fig. 3

From: Circular RNA F-circEA-2a derived from EML4-ALK fusion gene promotes cell migration and invasion in non-small cell lung cancer

Fig. 3

Identification of F-circEA-2a in NSCLC patients’ samples. a Primers used to detect EML4-ALK mRNA and F-circEA-2a/4a. The convergent primers (F2/R2) were used to detect EML4-ALK mRNA, the divergent primers F3/R3 and F4/R4 were used to detect F-circEA-4a/2a, respectively. b.c Agarose gel electrophoresis and Sanger sequencing of RT-PCR products from tumor tissues (b) or plasma (c) of NSCLC patients with (patients 1–3) or without (patients 4–5) EML4-ALK variant 3b translocation. See Additional file 1:Table S1 for the information of the primers and oligonucleotides in this figure. The experimental protocols are described in the Additional file 2

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Molecular Cancer

ISSN: 1476-4598