Fig. 2

The biological structure of circ-KIAA1244. (a) Schematics showed that circ-KIAA1244 is derived from KIAA1244 exons 3–8. The amplification products were inserted into a T-vector for Sanger sequencing to determine their full-length. (b) qRT-PCR for the abundance of circ-KIAA1244 and KIAA1244 mRNA in GC cells treated with RNase R. (c) qRT-PCR for the abundance of circ-KIAA1244and KIAA1244 mRNA in GC cells treated with actinomycin D. (d) circ-KIAA1244 was mainly localized in the cytoplasm. (e) circ-KIAA1244 was enriched in Ago2-containing immunoprecipitates compared with control immunoglobulin G (IgG) immunoprecipitates. (f) The possible binding miRNAs and mRNAs of circ-KIAA1244. **P<0.01