Fig. 3

miR-429 represses TIF1γ expression by targeting 3’-UTR of TIF1γ transcript. a Schematic description for the subcloning of the predicted miR-429 binding sites of TIF1γ 3’-UTR in psiCHECK-2 luciferase vector. Predicted duplex formation between miR-429 and the wild-type/mutant of miR-429 binding sites was indicated. The entire subcloning sequences were listed in Additional file 4: Table S2. b Relative luciferase activity of the wild-type/mutant TIF1γ 3’-UTR reporter gene in A549 and H226 cells transfected with miR-429 or negative control (miR-NC). Scrambled sequence was used as miR-NC. Relative Renilla luciferase activity was determined after normalizing against the firefly luciferase activity. c qRT-PCR analysis of miR-429 expression levels in A549 and H226 cells transfected with miR-429 mimics or miR-NC. U6 was employed as internal control. d, e TIF1γ mRNA and protein expression in A549 and H226 cells transfected with miR-429 mimics or miR-NC. β-actin was used as internal control. f miR-429 expression levels in A549 and H226 cells transfected with miR-429 inhibitors (anti-miR-429) or negative control (anti-miR-NC). Scrambled sequence was used as anti-miR-NC. g, h TIF1γ mRNA and protein expression in A549 and H226 cells transfected with anti-miR-429 or anti-miR-NC. *P < 0.05; ***P < 0.001