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Fig. 1 | Molecular Cancer

Fig. 1

From: Proteogenomic characterization and comprehensive integrative genomic analysis of human colorectal cancer liver metastasis

Fig. 1

Mass-spectrometry-based proteomics workflow. Protein was extracted from fresh CRC and paired PN tissues and was used to generate tryptic digests. The resulting tryptic peptides were fractionated using off-line bRPLC Collected fractions were pooled and used with a Thermo Orbitrap-Velos MS instrument. Raw data were processed by Perseus software and then used for database and spectral library evaluation using the Andromeda peptide search engine. Identified peptides were assembled using Maxquant software. bRPLC, basic reverse-phase (high-pressure) liquid chromatography; CRC, colorectal cancer; PN paracarcinoma normal tissue

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Molecular Cancer

ISSN: 1476-4598