Fig. 2
From: Circular RNA BCRC-3 suppresses bladder cancer proliferation through miR-182-5p/p27 axis
Enforced expression of BCRC-3 inhibits cell proliferation in vitro and impairs tumor growth in vivo. a qRT-PCR analysis verified the effective overexpression of BCRC-3 after transfection of the vector for 48 h in EJ or T24 T cells. b Cell cycle distributions in BCRC-3 overexpression cells were presented by flow cytometry. c Cloning formation assay of EJ or T24 T cells transfected with BCRC-3 overexpression vectors. Colonies with more than 50 cells were counted. d & e Observation of DNA synthesis of EJ or T24 T cells transfected with BCRC-3 overexpression vectors by EdU assay. f T24 T cells with stable overexpression of BCRC-3 or vector were injected in the flanks (5 × 106cells per mouse, n = 6 for each group). Tumors collected from mice were measured and weighed after one month of hypodermic injection. (Data are mean ± SEM of three experiments. Student’s t-test analyzed the difference in a-c, e-f. * P < 0.01 vs. vector)