Fig. 5
From: Circular RNA BCRC-3 suppresses bladder cancer proliferation through miR-182-5p/p27 axis
MJ inhibits cell proliferation through BCRC-3/miR-182-5p/p27 axis in BC cells. a Western blot analysis of the expression levels of cell cycle related proteins (p27, CyclinE, p21) in EJ or T24 T cells at different time points after MJ treatment. b Western blot analysis of the expression levels of p27 after MJ treatment in cells that were stably transfected with shP27 cells. c & d Cell cycle distributions in p27 knockdown cells after MJ treatment were presented by flow cytometry. e qRT-PCR analysis of the expression levels of p27 in EJ or T24 T cells after MJ treatment for different time points. f & g The luciferase activities of p27 3’UTR and promoter in EJ or T24 T cells after MJ treatment for 24 h. h & i qRT-PCR analysis of the expression levels of BCRC-3 (h) and miR-182-5p (i) in EJ or T24 T cells after MJ treatment for different time points. j & k Western blot analysis of the expression levels of p27 after MJ treatment in EJ or T24 T cells transfected with miR-182-5p mimics or si BCRC-3. (The data indicated mean ± SEM of three experiments. Student’s t-test analyzed the difference in d-i. & P < 0.05 vs. shNC + DMSO. # P < 0.05 vs. shP27 + DMSO. * P < 0.01 vs. control)