Fig. 2

Gastric cancer cell-derived conditioned medium induced pro-tumor activation of neutrophils through NF-κB pathway. a. Western blot assays for the expression of p-p65, p-STAT3, and p-ERK in neutrophils treated with GC-CM for different times. b-e. Neutrophils were pre-treated with inhibitors for NF-κB, STAT3, and ERK pathways followed by incubation with BGC-CM. The expression of phosphorylated NF-κB, STAT3, and ERK in neutrophils was determined by western blot (b). Western blot assays for LC3-II expression in neutrophils (c). QR-PCR analyses of ATG7 and BECN1 expression in neutrophils (d). Gastric cancer cells were incubated with the supernatants from neutrophils and used for transwell migration assay (e). ^**P<0.01 and ^*P<0.05 compared to control; ^##P<0.01 and ^#P<0.05 compared to BGC-CM