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Fig. 6 | Molecular Cancer

Fig. 6

From: Tumor-derived exosomes induce N2 polarization of neutrophils to promote gastric cancer cell migration

Fig. 6

Gastric cancer tissue-derived exosomes induced pro-tumor activation of neutrophils. a. In silicon analyses of HMGB1 expression in paired gastric cancer and non-cancerous tissues from GEO dataset (GSE13911). b. The expression of HMGB1 in gastric cancer tissues and paired non-cancerous tissues was measured by ELISA (n=9). c. Tissue microarray analyses of HMGB1 expression in paired gastric cancer tissues and non-cancerous tissues (n=76). Survival time of gastric cancer patients was analyzed by Kaplan-Meier analysis. d-e. The percentage of apoptotic cells (d) and CD11b expression (e) in neutrophils treated with gastric cancer tissue-derived exosomes (T-Ex) and non-cancerous tissue-derived exosomes (N-Ex) were determined by flow cytometry. f. QRT-PCR analyses of ATG7 and BECN1 expression in neutrophils treated with T-Ex and N-Ex. g. Western blot assays for the activation of NF-κB, STAT3, and ERK pathways in neutrophils treated with T-Ex and N-Ex. h. Gastric cancer cells were incubated the supernatants from T-Ex- and N-Ex-treated neutrophils and used for transwell migration assay. i-k. Neutrophils were treated with T-Ex in the presence or absence of HMGB1 antagonist (Gly). The expression of ATG7 and BECN1 (i) and inflammatory factors (j) is measured by qRT-PCR. k. Transwell migration assays for gastric cancer cells following treatment with supernatant from neutrophils. *P<0.05 compared to control (Ctrl); ##P<0.01 and #P<0.05 compared to T- Ex

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