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Fig. 7 | Molecular Cancer

Fig. 7

From: Tumor-derived exosomes induce N2 polarization of neutrophils to promote gastric cancer cell migration

Fig. 7

HMGB1 knockdown impaired neutrophil activation by gastric cancer cell-derived exosomes. HMGB1 was silenced in gastric cancer cell by siRNA. Neutrophils were treated with exosomes from HMGB1-silenced (si-HMGB1) and scramble control siRNA (si-Scr) transfected gastric cancer cells. a. Western blot assays for HMGB1 expression in gastric cancer cells transfected with HMGB1 siRNA and their derived exosomes. b-c. The percentage of apoptotic cells (b) and CD11b expression (c) in neutrophils were determined by FACS analyses. d. Western blot assays for LC3-II expression in neutrophils. e. The expression of ATG7 and BECN1 genes is measured by qRT-PCR. f. The expression of p-p65, p-STAT3 and p-ERK in neutrophils was determined by western blot. g. The expression of inflammatory factors in neutrophils was measured by qRT-PCR. h. Gastric cancer cells was incubated with the supernatants from neutrophils and subjected to transwell migration assay. i. Proposed model for the role of tumor-derived exosomes in the pro-tumor activation of neutrophils to promote gastric cancer cell migration. **P<0.01 and *P<0.05 compared to control (Ctrl); ##P<0.01 and #P<0.05 compared to si-Scr Ex

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