Fig. 4
From: Regulation of Sox2 and stemness by nicotine and electronic-cigarettes in non-small cell lung cancer
a Nicotine and E-cigarette extracts promote the co-localization of E2F1 and YAP1 in A549 cells, as seen by a double immunofluorescence experiment followed by confocal microscopy. b An immunoprecipitation-western blot experiment showing the association of YAP1 with E2F1 in A549 and H1650 cells; the immunoprecipitation was conducted by an E2F1 antibody followed by western blotting with a YAP1 antibody. c An IP-western blot experiment in the reverse direction, where a YAP1 antibody was used for IP followed by western blotting with an E2F1 antibody, further confirms the association of YAP1 with E2F1. IP with an antibody to Rb was used as a positive control in this experiment. d A proximity ligation assay showing the enhanced association of YAP1 with E2F1 in A549 cells upon treatment with nicotine or E-cigarette extracts. The interaction was maximal at 24Â h