Fig. 5
From: Regulation of Sox2 and stemness by nicotine and electronic-cigarettes in non-small cell lung cancer
a Treatment with selected inhibitors prevents nicotine-mediated induction of Sox2; the Src family kinases appear to be especially vital for the induction. b Depletion of Src, Yes or β-arrestin-1 reduces the nicotine-mediated induction of Sox2 in A549 cells (c) Treatment with a siRNA to Src reduces YAP1 levels and its interaction with E2F1; similar results were obtained upon treatment with a Src inhibitor (d). e Proximity ligation assay showing that depletion of Src abrogates the interaction of YAP1 with E2F1; similar results were obtained upon treatment with a Src inhibitor (f). g An IP-western blot experiment showed that depeltion of Yes and perhaps Src reduces the icotine-mediated interaction of YAP1 with E2F1