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Fig. 6 | Molecular Cancer

Fig. 6

From: Regulation of Sox2 and stemness by nicotine and electronic-cigarettes in non-small cell lung cancer

Fig. 6

a Nicotine can induce the association of Oct4 with YAP1 in A549 cells, as seen by a proximity ligation assay. b Depletion of Oct4, E2F1 or YAP1 prevents the nicotine-mediated induction of Sox2-Luc in A549 and H1650 cells. c Similarly, mutating Oct4 or E2F binding sites on the Sox2 promoter prevents its induction by nicotine in A549 cells, as seen in a transient transfection experiment. d Mutating Oct4 binding sites prevents the induction of Sox2-Luc by Oct4, but the promoter is responsive to E2F1; conversely, mutating E2F binding site 1 or site 2 prevents the induction of the Sox2-Luc reporter by E2F1, but conserves the response to Oct4 in a transient transfection experiment. The graphical data represented in this figure has ± standard deviation (SD) values derived from three independent experiments. The statistical comparisons between the representative groups were carried out by one-way ANOVA to determine the statistical significance. e Schematic representing the nicotine mediated upregulation of Sox2. Nicotine binds to the α7 nAChR receptor and activates Src in a βArr1 dependent manner which promotes the binding of to Oct4 and E2F1, resulting in the induction of Sox2

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