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Fig. 2 | Molecular Cancer

Fig. 2

From: High expression of Tob1 indicates poor survival outcome and promotes tumour progression via a Wnt positive feedback loop in colon cancer

Fig. 2

Tob1 promotes colon cancer cell proliferation via a Wnt/β-catenin signalling feedback loop. a Tob1 expression analysis by qRT-PCR and WB. b-c Effects of Tob1 knockdown or overexpression on cell proliferation analysed by MTT. d Wnt activity analysis by the TOPFlash/FOPFlash assay. e mRNA expression analysis of Wnt/β-catenin target genes by qRT-PCR. f β-Catenin levels in cells treated with CHX (10 μg/ml). g Subcellular localization analysis of Tob1 by nucleocytoplasmic separation. h Interaction analysis of β-catenin and Tob1 by co-IP. i β-Catenin expression analysis by WB. j β-Catenin regulated the expression but not phosphorylation of Tob1. k Tob1 expression was upregulated by LiCl in RKO cells. *, P < 0.05; **, P < 0.01 and ***, P < 0.001 vs control

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Molecular Cancer

ISSN: 1476-4598