Fig. 3

circFNDC3B exerts anti-tumor effects in BC cells. a Schematic illustration of two targeted siRNAs. si-circFNDC3B targets the back-splice junction of circFNDC3B. b and c qRT-PCR analysis of circFNDC3B and FNDC3B mRNA in T24 cells treated with siRNAs (b) and UM-UC-3 cells stably overexpressing circFNDC3B (c). d and e The cell proliferative ability was assessed by MTS and colony formation assay after knocking down circFNDC3B in T24 cells. (f-g) Cell migratory and invasive capabilities were assessed by wound healing assay and transwell assay after knocking down circFNDC3B in T24 cells. (h-i) The cell proliferative ability was assessed by MTS and colony formation assay after overexpressing circFNDC3B in UM-UC-3 cells. (j-k) The cell migration and invasion capabilities were assessed by wound healing assay and transwell assay after overexpressing circFNDC3B in UM-UC-3 cells. The red scale bar indicates 200 μm; the black scale bar indicates 100 μm. Data are presented as the means±SEM of three experiments. *P < 0.05, **P < 0.01(Student’s t-test)