Fig. 4

circFNDC3B could serve as a miR-1178-3p sponge in BC cells. a Potential target miRNAs of circFNDC3B were predicted by CircInteractome. b and c RNAs pulled down by a circFNDC3B probe were tested by qRT-PCR (b) and gel electrophoresis (c). GAPDH was used as a negative control. Relative levels of circFNDC3B were normalized to input. d qRT-PCR analysis of miRNA candidates in T24 and UM-UC-3 lysates. e The Renilla luciferase activity of wild type or mutant circFNDC3B in the miR-1178-3p mimics or NC group. f The Renilla luciferase activity analysis in 293 T cells after transfection with miR-1178-3p mimics+psiCHECK-2 empty vector, miR-1178-3p mimics+wild type circFNDC3B or miR-1178-3p mimics+mutant circFNDC3B. Data are presented as the ratio of Renilla luciferase activity to firefly activity. g The RNAs captured by biotinylated wild-type or mutant miR-1178-3p in T24 and UM-UC-3 cells were quantified by qRT-PCR. h Colocalization of circFNDC3B and miR-1178-3p was conducted by FISH. Nuclei were stained with Hoechst33342. Scale bar, 50 μm. Data are presented as the means±SEM of three experiments. *P < 0.05, **P < 0.01 (Student’s t-test)