Fig. 9
Overexpression of circFNDC3B suppresses the growth and lymphatic metastasis of BC cells in vivo. a Image of BALB/c nude mice injected with UM-UC-3 cells subcutaneously (5 × 106 cells per mouse, n = 5 for each group). b Analysis of tumor volume of mice measured every week. c Image of subcutaneous xenograft tumors. Tumor weights were significantly decreased in circFNDC3B–treated mice. d IHC of G3BP2, p-SRC, p-FAK in the subcutaneous tumors. Scale bar, 200 μm. e Representative image of metastatic popliteal LN captured by an in vivo bioluminescence imaging system. f The image of excised popliteal LNs. The LN volume significantly decreased in circFNDC3B-treated mice. g HE staining of popliteal LNs. Scale bar, 200 μm. Data indicate the means±SEM of three experiments, *P < 0.05, **P < 0.01 (Student’s t-test)