Fig. 8

CircSLC3A2 acted as a sponge of miR-490-3p in HCC cells. a Schematic representation of potential binding sites of miR-490-3p with WT or Mut circSLC3A2. b qRT-PCR analysis of the expression level of miR-490-3p after transfection with circSLC3A2 in HepG2 cells or sh-circSLC3A2 in LO2 cells. c The luciferase activity of WT or Mut circSLC3A2 after co-transfection with pMIR-Luc-circSLC3A2 and miR-490-3p mimic or inhibitor in HepG2 or LO2 cell line. d Ago2 RIP assay for the amount of circSLC3A2 and miR-490-3p in HepG2 or LO2 cell line. e qRT-PCR and Western blot analysis of the expression levels of PPM1F after co-transfection with circSLC3A2 and miR-490-3p mimic in HepG2 cells or sh-circSLC3A2 and miR-490-3p inhibitor in LO2 cells. f MTT analysis of the cell viability after co-transfection with circSLC3A2 and miR-490-3p mimic in HepG2 cells or sh-circSLC3A2 and miR-490-3p inhibitor in LO2 cells. Data are the means ± SEM of three experiments. *P < 0.05; **P < 0.01