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Fig. 6 | Molecular Cancer

Fig. 6

From: EIF4A3-induced circular RNA MMP9 (circMMP9) acts as a sponge of miR-124 and promotes glioblastoma multiforme cell tumorigenesis

Fig. 6

eIF4A3 regulates circMMP9 expression. a The binding sites of eIF4A3 were predicted in the upstream region of the MMP9 mRNA transcript using the circRNA interactome. b The RIP assay was performed to verify eIF4A3 binding with MMP9 mRNA. H19 lncRNA was used as the positive control, while intron 11 of MMP9 was used as the negative control. qRT-PCR was used to detect the transcript abundance relative to input. c A schematic diagram of 5 RNA constructs, which are truncated to varying degrees (a1-a5) and contain the eIF4A3 binding sites, is shown. Laz was a non-sense sequence used as the negative control, and H19 was used as the positive control. The RNA pull-down assay was performed to analyze the interaction between eIF4A3 and MMP9 mRNA (a1-a5). d U87 and U251 cells were transfected with control or eIF4A3 knockdown plasmid, and circMMP9 expression was detected by qRT-PCR. e U87 and U251 cells were transfected with control or eIF4A3 overexpression plasmid, and circMMP9 expression was measured by qRT-PCR

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