Skip to main content
Fig. 1 | Molecular Cancer

Fig. 1

From: The pro-metastasis effect of circANKS1B in breast cancer

Fig. 1

Identification of circular RNAs by RNA-seq and the characteristics of circANKS1B in breast cancer. a Heat map and hierarchical clustering analysis of the top ten up-regulated and down-regulated circRNAs in TNBC compared with adjacent normal tissues. Red colour represents high expression level, and blue colour represents low expression level. b qRT-PCR analysis of the expression of circANKS1B in twenty pairs of TNBC and adjacent normal tissues. c The genomic loci of ANKS1B gene and circANKS1B. Red arrow indicates the back-splicing of ANKS1B exon 5 to exon 8 confirmed by Sanger sequencing. The spliced mature sequence length of circANKS1B is 459 bp. d The schematic illustrates the targeted regions for qRT-PCR primers (Divergent primers for detecting circANKS1B and convergent primers for detecting linear ANKS1B). e RT-PCR for the analysis of the existence of circANKS1B in breast cancer tissue and cells. Divergent primers detected circANKS1B in cDNA but not genomic DNA (gDNA). GAPDH was used as the negative control. f Fluorescence in situ hybridization (FISH) assay was carried out to determine the subcellular localization of circANKS1B. circANKS1B probe was labeled with Cy3 (red), nuclei were stained with DAPI (blue). Scale bar = 10 μm. g qRT-PCR for the expression of circANKS1B in 40 normal tissues and 165 breast cancer tissues. h Analysis of circANKS1B expression in breast cancer patients with or without lymph node metastasis. Using median circANKS1B value as cutoff. i Kaplan-Meier survival curves of breast cancer patients with low and high circANKS1B expression. Using median circANKS1B value as cutoff. **p < 0.01, ***p < 0.001

Back to article page