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Fig. 3 | Molecular Cancer

Fig. 3

From: LncRNA MIR31HG targets HIF1A and P21 to facilitate head and neck cancer cell proliferation and tumorigenesis by promoting cell-cycle progression

Fig. 3

MIR31HG targeted HIF1A and p21 to regulate the cell cycle progression in HNSCC cells. a PI-FACS analysis of cell cycle distribution in FaDu and Cal-27 cells after MIR31HG shRNA transfection. Representative plots and cell percentage at different phases are illustrated. b Annexin V-APC-FACS analysis of cell apoptosis in FaDu and Cal-27 cells after MIR31HG shRNA transfection. Representative scatter plots and quantitative results are shown. c Differential mRNA expression profiling in MIR31HG KD and NC groups. Red color indicated overexpression and green color indicated low expression. Every column represented a tissue sample, and every row represented a mRNA probe. d Upper panel was the summary of ingenuity pathway analysis (IPA) biofunctions related to MIR31HG knockdown (fold-change ≥ 1.5, FDR < 0.05). Lower panel was p53 pathway heatmap indicated altered gene expression in p53 pathway after the MIR31HG knockdown. e Western blot of HIF1A and HIF1A-regulated targets in FaDu (left) and Cal-27 (right) cells treated with MIR31HG shRNA. *P < 0.05, **P < 0.01 by Student’s t-test

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Molecular Cancer

ISSN: 1476-4598