Fig. 2
From: CircFAT1 sponges miR-375 to promote the expression of Yes-associated protein 1 in osteosarcoma cells
Knockdown of circFAT1 inhibits the migration and invasion of osteosarcoma cell lines in vitro. a The expression levels of circFAT1 and FAT1 mRNA in HOS and 143B cells after stable transfection of circFAT1 short hairpin RNAs or vector plasmids were detected by real-time PCR. Data represents the mean ± SD (n = 3). * P < 0.05. b ShRNA-mediated circFAT1 knockdown suppressed OS cell proliferation, as determined in the CCK-8 assay. Data represents the mean ± SD (n = 6). c HOS and 143B cells were transfected with shcircFAT1, followed by Annexin V-FITC/PI staining. The percentage of apoptotic cells is shown as the mean ± S.D. from the three independent experiments. * P < 0.05, significantly different compared with the vector group. d CircFAT1 knockdown suppresses cell growth, as determined by the colony formation assay (details are shown in the insets). Error bars represent the mean ± SD of three independent experiments. * P < 0.05. e Cell migration and invasion abilities of HOS and 143B cells transfected with shcircFAT1 or vector were evaluated by Transwell migration and Matrigel invasion assays. Data represents the mean ± SD (n = 3). * P < 0.05. Scale bar, 50 μm. f The effect of shcircFAT1 on cell migration capability was evaluated by the wound healing assay in HOS and 143B cells. Scale bar, 200 μm