Fig. 4

MiR-375 is associated with OS cell migration and invasion. a miR-375 expression was lower in human OS than in chondroma tissue. Data represent the mean ± SD (n = 12). b miR-375 expression was lower in human OS than in chondroma tissue. Representative images are shown (Scale bars = 100 μm or 50μm). c miR-375 expression in hFOB1.19 and OS cell lines (OS-732, 143B, HOS, SJSA-1, and MG-63) was evaluated by qRT-PCR. Data represents the mean ± SD (n =3). * P < 0.05 (d) Pre-miR-375-mediated miR-375 overexpression suppressed OS cell proliferation, as determined by the CCK-8 assay. Data are presented as the mean ± SD (n = 6). e HOS and 143B cells were transfected with miR-375 sponge, followed by Annexin V-FITC/PI staining. The percentage of apoptosis cells is shown as the mean ± SD from three independent experiments. * P < 0.05, significantly different compared with the vector group. f miR-375 overexpression suppresses cell growth, as determined by the colony formation assay (details are shown in the insets). Error bars represent the mean ± SD of three independent experiments. * P < 0.05. g Cell migration and invasion of HOS and 143B cells, transfected with pre-miR-375 or vector, were evaluated by Transwell migration and Matrigel invasion assays. Data represent mean ± SD (n = 3). * P < 0.05. Scale bar, 50 μm. h The effect of pre-miR-375 on cell migration capability was evaluated by a wound healing assay in HOS and 143B cells, respectively. Data represents the mean ± SD (n = 3). * P < 0.05. Scale bar, 200 μm