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Fig. 4 | Molecular Cancer

Fig. 4

From: C-Src confers resistance to mitotic stress through inhibition DMAP1/Bub3 complex formation in pancreatic cancer

Fig. 4

Bub3/DMAP1 complex represses anti-apoptotic genes transcription. In a, immunoblotting analyses were performed using the indicated antibodies; data represent 1 out of 3 experiments. In c-e, the values represent mean ± s.e.m. of three independent experiments. a, SW1990 cells were double blocked by thymide and treated with nocodazole (200 nM) following by releasing for the indicated periods. b, SW1990 cells were released for 4 h after thymidine double block and nocodazole (200 nM) for 16 h. Hierachical clustering of 4307 probe sets correlating with DMAP1 Y246F-expressed cells show that genes relevant to anti-apoptosis or autophagy were effective in separating cases from DMAP1 WT-expressed cells. c and d SW1990 cells expressed with the indicated plasmids were treated with nocodazole (200 nM) post thymidine double block, and were released for the indicated time. Relative mRNA levels were analyzed by real-time PCR. In c, * represents p < 0.05 between groups of cells expressing rDMAP1 Y246F plus WT rBub3 and groups of cells expressing rDMAP1 Y246F plus rBub3 S211A. In d, * represents p < 0.05 between groups of cells expressing rDMAP1 Y246F and groups of cells expressing rDMAP1 Y246F plus DNMT1 siRNA. e, Cell apoptosis was analyzed by Annexin V assays followed by flow cytometry. ** represents p < 0.01 between indicated groups

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