Fig. 1

Ectopic expression of LINC00312 promoted metastasis and VM of ADC cells. a Boxplot of LINC00312 expression from 124 ADC patients. High LINC00312 expression was positively correlated with TNM metastasis. b The quantitation of lung metastasis was assessed by bioluminescence measurements. The bioluminescent change in LINC00312-OE group was significantly increased compared with the control. c Hematoxylin and eosin (HE) staining in the lung samples obtained from mice after injection with stable pIRES2-LINC00312 PC-9 cells. d and e Schematic presentation of the ability of ADC cells form VMs. Bar charts showing number of nodes, number of branches, Mash size and Mash area in stable. Quantification from three independent replicates is shown on the right. f Typical images of CD31-/PAS+ double staining in the lung samples obtained from mice after injection with stable pIRES2-LINC00312 PC-9 cells. Control represent wild cells transfected with vector plasmid. Error bars represent SD from three reactions. Significance was calculated using Student’s t test. *p < 0.05; **p < 0.001; ***p < 0.0001; ns, not significant. Scale bars: (c, d, e)100μm in 100 x images, (f) 200μm in 100x and 400x images