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Fig. 2 | Molecular Cancer

Fig. 2

From: TRIB2 functions as novel oncogene in colorectal cancer by blocking cellular senescence through AP4/p21 signaling

Fig. 2

Knockdown of TRIB2 in CRC cells inhibits tumor cell growth and enhances cellular senescence. a Western blot of TRIB2 in SW48 and LoVo cells transfected with TRIB2-specific siRNA (siTRIB2#1 and siTRIB2#2) or control siRNA. b CCK8 analysis of cell viability in TRIB2-knockdown SW48 and LoVo cells at 0, 24, 48 and 72 h, respectively. c Cell cycle distribution measured by flow cytometry in TRIB2-knockdown SW48 and LoVo cells. d SA-β-gal staining analysis in TRIB2-knockdown SW48 and LoVo cells treated with dox (0.25 μmol/l, 48 h, left panel, representative images of SA-β-gal staining). e Western blot of TRIB2, p53 and p21 in SW48 and LoVo cells transfected with TRIB2-specific or control siRNA. f mRNA expression of p53 and p21 in SW48 and LoVo cells transfected with TRIB2-specific or control siRNA. g Luciferase activity of p21 promoter in SW48 and LoVo cells transiently transfected with p21-Luc plus TRIB2-specific or control siRNA. Results are presented as mean ± SD from three independent assays, * p < 0.05, ** p < 0.01, *** p < 0.001, t-test

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