Fig. 5

TRIB2 regulates cell proliferation and cellular senescence dependent on AP4. a Western blot analysis of TRIB2, AP4 and p21 expression in SW48 and LoVo cells transfected with TRIB2-expressing plasmid and or not AP4-specific siRNA. b mRNA expression of p21 in SW48 and LoVo cells transfected with TRIB2-expressing plasmid and or not AP4-specific siRNA. c p21-Luc was co-transfected with TRIB2-expressing plasmid and or not AP4-specific siRNA into SW48 and LoVo cells. After 48 h of transfection, the cells were harvest and luciferase activities were measured. d Western blot of TRIB2, AP4 and p21 expression in SW48 and LoVo cells transfected with AP4-expressing plasmid and or not TRIB2-specific siRNA. e mRNA expression of p21 in SW48 and LoVo cells transfected with AP4-expressing plasmid and or not TRIB2-specific siRNA, respectively. f p21-Luc was co-transfected with AP4-expressing plasmid and or not TRIB2-specific siRNA into SW48 and LoVo cells. After 48 h of transfection, the cells were harvest and luciferase activities were measured. g Cell proliferation analysis of TRIB2-overexpressed SW48 and LoVo cells transfected with or without AP4-specific siRNA. h SA-β-gal staining analysis in TRIB2 overexpressed SW48 and LoVo cells transfected with or without AP4-specific siRNA (dox, 0.25 μmol/l, 48 h). The percentage of SA-β-gal-positive cells was analyzed (right panel). i Cell proliferation analysis of AP4-overexpressed SW48 and LoVo cells transfected with or without TRIB2-specific siRNA. j SA-β-gal staining analysis in AP4-overexpressed SW48 and LoVo cells transfected with or without TRIB2-specific siRNA (dox, 0.25 μmol/l, 48 h). The percentage of SA-β-gal-positive cells was analyzed (right panel). Results are presented as mean ± SD from three independent assays, * p < 0.05, ** p < 0.01, *** p < 0.001, t-test