Fig. 4

BMP5 inhibits cell proliferation in CRC model. a-b HT-29 and SW480 cell proliferation was determined by CCK8 assays after transfection, on day 1, 2, 3, 4. c-d Cell cycle distributions of HT-29 and SW480 cells were detected by flow cytometry. Representative of triplicate experiments was shown. e Images of isolated tumors from injected nude mice. f Tumor volume was assessed every 4 days with a total period of 25 days (n = 6). g Weights of the isolated tumor (n = 6). All data are showed as mean ± sem. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001