Fig. 8

Silence of TINCR impairs trastuzumab resistance and metastasis in vivo. a-b Representative images of the nude mice and corresponding xenograft after intraperitoneal injection with once every two days 3 mg/kg trastuzumab for three weeks in sh-NC or sh-TINCR cell groups (five mice in each group). c Weight of tumors that developed in xenografts from each group is shown, ^**P < 0.01. d Lung metastases were quantified using bioluminescence imaging after 5 weeks of initial implantation. Representative in vivo bioluminescent images (left) and quantitative photon flux (right) are shown, ^***P < 0.001. e Representative images of metastatic tumor nodules (left) and corresponding quantitative number of metastasis (right), ^***P < 0.001. f The expression levels of TINCR, HER-2, and Snail-1 in implanted breast cancer tissues were measured by qPCR. g Representative images of HER-2 and Snail-1 expression in the implanted breast cancer tumors in each group as analyzed by immunohistochemical