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Fig. 3 | Molecular Cancer

Fig. 3

From: The circRNA circAGFG1 acts as a sponge of miR-195-5p to promote triple-negative breast cancer progression through regulating CCNE1 expression

Fig. 3

circAGFG1 promotes TNBC cell proliferation. a The schematic illustration of circAGFG1 expression vector and shRNAs. b and c qRT-PCR analysis of circAGFG1 and AGFG1 RNA expression in TNBC cells transfected with circAGFG1 expression vector, mock, sh-circ or sh-NC. d The growth curves of cells transfected with indicated vectors were evaluated by CCK8 assays. e and f EdU assays were conducted in cells after transfection with indicated plasmids (magnification, × 100). Scale bar, 100 μm. g and h Colony formation assays were executed to detect the proliferation of cells transfected with indicated vectors. Data were showed as mean ± SD, *P < 0.05, **P < 0.01, ***P < 0.001, N.S, nonsignificant

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