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Fig. 7 | Molecular Cancer

Fig. 7

From: The circRNA circAGFG1 acts as a sponge of miR-195-5p to promote triple-negative breast cancer progression through regulating CCNE1 expression

Fig. 7

CCNE1 is directly targeted by miR-195-5p and indirectly regulated by circAGFG1. a Schematic illustration of CCNE1 3’UTR-WT and CCNE1 3’UTR-Mut luciferase reporter vectors. b The relative luciferase activities were detected in 293 T cells after transfected with CCNE1 3’UTR-WT or CCNE1 3’UTR-Mut and miR-195-5p mimics or miR-NC, respectively. c and d Relative mRNA and protein levels of CCNE1 were detected in cells after transfected with miR-NC, miR-195-5p, ihn-NC and inh-195-5p using qRT-PCR and western blot, respectively. e and f Relative expression of CCNE1 was detected by qRT-PCR in cells transfected with indicated vectors, miRNAs or inhibitors. Data were indicated as mean ± SD, *P < 0.05, **P < 0.01, ***P < 0.001

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