Fig. 3

CircNRIP1 serves as a miRNA sponge of miR-149-5p. a We selected the top 10 most common miRNAs based on their conjugation scores, which might indicate potentially functional miRNAs absorbed by circNRIP1 in GC cells. b. We confirmed the enrichment of circNRIP1 and miR-149-5p in MKN-45 and BGC-823 cells. c. We used a specific biotin-labelled miR-149-5p probe to successfully capture circNRIP1 relative to the NC group. d. We performed a dual-luciferase reporter assay to determine the direct binding between circNRIP1 and miR-149-5p based on their complementary sequences. e. We found that miR-149-5p co-localized with circNRIP1 in the cytoplasm by FISH analysis, scale bar = 100 μm. f. We determined the negative correlation between the expression levels of circNRIP1 and miR-149-5p by performing qRT-PCR on 80 paired GC tumour tissues and normal tissues. (g, h). We confirmed that the low level of miR-149-5p was positively correlated with the OS (median survival of 58 months vs 21 months; P = 0.0007, log-rank test) and DFS (median survival of 56 months vs 19 months; P = 0.0002, log-rank test) of GC patients. All data are presented as the mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001