Fig. 5

Knockdown of circNRIP1 inhibits the proliferation, migration and invasion of GC cells in vitro. a. We observed that circNRIP1 silencing significantly inhibited the cell proliferation rate, as indicated by the CCK8 assay, and overexpression of circNRIP1 exerted the opposite effect on the GC cell proliferation rate. b. We observed that circNRIP1 silencing significantly inhibited DNA synthesis, as determined by the Edu assay, and overexpression of circNRIP1 exerted the opposite effect on GC cell DNA synthesis as indicated by the EDU assay, scale bar = 100 μm. c. We observed that circNRIP1 silencing significantly inhibited colony formation ability, and overexpression of circNRIP1 exerted the opposite effect on colony formation. d. Knockdown of circNRIP1 successfully reduced the migration and invasion ability of GC cells, and overexpression of circNRIP1 exerted the opposite effect on metastasis, as indicated by the transwell assay, scale bar = 200 μm. e. We observed that both circNRIP1 knockdown and miR-149-5p overexpression significantly inhibited the growth of the organoid models, while circNRIP1 overexpression promoted organoid model survival. f. We detected downregulation of the mesenchymal cell markers TWIST, snail, slug, and N-cadherin and upregulation of the epithelial cell marker E-cadherin in human GC organoid models when we knocked down circNRIP1 or overexpressed miR-149-5p. All data are presented as the mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001