Fig. 1

Expression pattern of circRNAs by qRT-PCR in plasma and exosome from cell culture conditioned media. a~c The expression levels of 3 circRNAs were detected by qRT-PCR in plasma from 50 ESCC patients and 50 healthy people. Student’s t-test was used for differential analysis and data were presented as the means± SEM. d Receiver operating characteristic (ROC) curve analysis of differentially expressed circRNAs between ESCC patients and healthy controls. The signature was a combination of hsa_circ_0043603 and hsa_circ_0001946. The AUC, sensitivity, and specificity values were given in the graph. AUC: Area Under The Curve; 0043603: hsa_circ_0043603; 0001946: hsa_circ_0001946; signat:signature. e~f The expression levels of hsa_circ_0001946 and hsa_circ_0043603 were detected by qRT-PCR in exosome from cell culture conditioned media of Eca109, Eca9706 and Het-1A. One-way ANOVA was used for differential analysis and data were presented as the means± SEM